URL: http://www.addgene.org/47322
Proper Citation: RRID:Addgene_47322
Insert Name: Cas9
Organism: Other
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24873830
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, CRISPR, xenopus and zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Please see the Schier lab website http://labs.mcb.harvard.edu/schier/ for detailed protocols about target site selection, sgRNA production, stop codon cassette design, Cas9 protein purification, injection and downstream analysis. This plasmid was used to prepare and inject Cas9 mRNA into zebrafish zygotes.
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Source: Addgene