URL: http://www.addgene.org/36939
Proper Citation: RRID:Addgene_36939
Insert Name: DNMT1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16682412
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI restriction site (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917. The full-length cDNA for human DNMT1 was cloned into EcoRI and NotI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen.
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Source: Addgene