URL: http://www.addgene.org/33348
Proper Citation: RRID:Addgene_33348
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14702347
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:Rc/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The polylinker region of the pRc/CMV eukaryotic expression vector (Invitrogen) was cut with HindIII and XbaI, and the following annealed oligonucleotide was inserted: 5′-AGCTCCACCATGGACTACAAGGACGACGATGACAAGCATATGTGATGAAGCTT-3′ (underlined bases are restriction sites for NcoI, NdeI, and HindIII) and 5′-CTAGAAGCTTCATCACATATGCTTGTCATCGTCGTCCTTGTAGTCCATGGTGG-3′ (underlined bases are restriction site for XbaI). The HindIII restriction site was not regenerated and was reintroduced downstream of the FLAG sequence (DYKDDDDK).
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Source: Addgene