URL: http://www.addgene.org/26093
Proper Citation: RRID:Addgene_26093
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7746; Vector Backbone:p15TV-L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The p15TV-L vector (GenBank ID: EF456736.1) is a derivative of pET15b (Novagen) and is for infusion based cloning. It is used for T7 promoter driven expression of recombinant proteins with the addition of a 21 amino acid N-terminal fusion tag with 6X His followed by a TEV cleavage site. Two stop codons are included at C-terminal cloning site. Insertion of DNA sequence into the cloning/expression region is preformed using BD-Biosciences Infusion enzyme mediated directional recombination between complementary 15 nucleotide DNA sequences at the ends of the insert (PCR product) and BseRI linearized vector. Insertion of target sequence involves replacement of a SacB gene stuffer sequence, which provides for negative selection of the original plasmid on 5% sucrose. http://www.sgc.utoronto.ca/SGC-WebPages/toronto-vectors.php
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Source: Addgene