URL: http://www.addgene.org/20927
Proper Citation: RRID:Addgene_20927
Insert Name: Oct4 and Sox2; SV40LT and Klf4
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19325077
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10600; Vector Backbone:pCEP4; Vector Types:Episomal/EBNA; Bacterial Resistance:Ampicillin
Comments: Please note Addgene Quality Control sequence found mutation K278T in KLF4. Oct4 and Sox2 are coexpressed under a strong EF promoter. SV40LT and Klf4 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul. Addgene recommends screening 3-5 colonies immediately upon receipt, as this plasmid may be prone to recombination.
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Source: Addgene