URL: http://www.addgene.org/17923
Proper Citation: RRID:Addgene_17923
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMT-puro; Vector Types:Insect Expression, Drosophila metallothionein gene promoter drives expression; Bacterial Resistance:Ampicillin
Comments: Insect expression vector. This was the empty vector used in http://www.addgene.org/pubmed/17589500 This is NOT the vector by the same name described in the Castellino lab's 2008 Cytotechnology paper. Notes from the Sabatini lab on using this plasmid: They did a puromycin kill curve and ended up using 2 ug/mL of puromycin for selection for S2R cells. They used 5 mM CuSO4 to induce expression of their gene 24 hours before performing the IF experiments. The metallothionein promoter is described in this link: https://www.ncbi.nlm.nih.gov/pubmed/3125519
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Source: Addgene