URL: http://www.addgene.org/16442
Proper Citation: RRID:Addgene_16442
Insert Name: p53-binding site
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8242752
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pBluescript II SK + luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: PG13-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream. PG13 contains 13 copies of the p53-binding consensus sequence. Direct repeats of the oligonucleotide PG (5'-CCAGGCAAGTCCAGGCAGG-3') were used for the p53 binding sequences.
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Source: Addgene