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on page 1 showing 20 out of 2,013 results

Cite this (TSC Cat# SD01994, RRID:TSC_SD01994)

Source Database: TSC, catalog # SD01994
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2]; mpr1-1/mpr1-1 (pm-s, mp-r, wt HHT1,2,3 HHF2, VII)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo3 KO of HHT!,HHT2/HHF2,HHT3
Availability:
Reference:
Notes: Germline heteokaryon for all three H3 genes(major and minor) and H4II, neo cassette.It is mating type VI Note: now know there is a HHT4 which was still present. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01625, RRID:TSC_SD01625)

Source Database: TSC, catalog # SD01625
Genetic Background: mat1-2/mat1-2 (mat1-2, II)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8253268
Notes: All three B2086 clones were used extensively for crosses in the Frankel lab in the late 1980's and 1990's. Submitted by Joseph Frankel, University of Iowa

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Cite this (TSC Cat# SD02647, RRID:TSC_SD02647)

Source Database: TSC, catalog # SD02647
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:16908532, PMID:9343391
Notes: HA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02457, RRID:TSC_SD02457)

Source Database: TSC, catalog # SD02457
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K130R]; pm-r)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutation K130R
Availability:
Reference: PMID:11430834, PMID:12665578
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated K130R. no ubiquitination of H2AF/Z. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02145, RRID:TSC_SD02145)

Source Database: TSC, catalog # SD02145
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU-HA-TAP]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
Availability:
Reference: PMID:12356864
Notes: Rescue of GTU05XGTU06 knockout heterokaryons with pTTMG-HA TAP. GTU1 with HA+TAP tag into MTT1 locus. MTT1 promoter, grow with 0.2ug/ml CdCl in 1xSPP. 5’MTT1-GTU-HA-TAP-MTT1 3’ TAP tag from Eric Phzicky is: GTU-HA-6XHis-3Cprotease cleavage-S.Aureus protein A. Constuct pTTMG-HA used to rescue GTU5 xGTU06 KO heterokaryons. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01841, RRID:TSC_SD01841)

Source Database: TSC, catalog # SD01841
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E435D])
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo knockout of btu2 with bsr Macronucleus: BTU1 has only (E431,432,435D) no WT BTU2 is KO
Availability:
Reference: PMID:11864572, PMID:15254268
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,432, 435D). The strain was supposed to be pm-r, but the thawed line is pm-s. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01453, RRID:TSC_SD01453)

Source Database: TSC, catalog # SD01453
Genetic Background: cdaK-1/cdaK-1 (cdaK-1, VII)
Affected Genes: CDAK (cell division arrest K)
Genomic Alteration:
Availability:
Reference: PMID:18658256
Notes: Unequal fission-arrest caused by diagonal and incomplete fission zone (39°). Temperature-sensitive expression; fully penetrant at 39°. Submitted by Joseph Frankel, University of Iowa

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Cite this (TSC Cat# SD01362, RRID:TSC_SD01362)

Source Database: TSC, catalog # SD01362
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (mp-r, VII)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes
Availability:
Reference: PMID:15701804
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It mates with gl∆H3/H4 #4 strain. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02718, RRID:TSC_SD02718)

Source Database: TSC, catalog # SD02718
Genetic Background: mpr1-1/mpr1-1 (rpc5[3’neo3,HAc]; pm-r, VII)
Affected Genes: RPC5 (TTHERM_00094210)
Genomic Alteration: Macronucleus: HA tagged RPC5
Availability:
Reference: PMID:15470252
Notes: All Somatic copy of RPA5 was replaced with RPA5-HA construct, neo3, CU428background. RPA5 is the fifth largest subunit of pol I and III. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01842, RRID:TSC_SD01842)

Source Database: TSC, catalog # SD01842
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E435D]; pm-r, BTUI has(E431,435D), BTU2 is replaced by bsr1)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo knockout of btu2 with bsr Macronucleus: BTU1 has only (E431,435D) no WT BTU2 is KO
Availability:
Reference: PMID:10831613, PMID:11864572
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,435D) From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02084, RRID:TSC_SD02084)

Source Database: TSC, catalog # SD02084
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[A1G] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with A1G
Availability:
Reference: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-A1G. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD00228, RRID:TSC_SD00228)

Source Database: TSC, catalog # SD00228
Genetic Background: bcd1-1/bcd1-1; lcd1-1/lcd1-1 (bcd1-1; lcd1-1; bcd1, lcd1, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes:

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Cite this (TSC Cat# SD01419, RRID:TSC_SD01419)

Source Database: TSC, catalog # SD01419
Genetic Background: mpr1-1/mpr1-1 (pm-r, VII)
Affected Genes: MTT1 (TTHERM_00241640)
Genomic Alteration: Macronucleus: Neo coding replaces MTT1 coding of some of MTT1 copies, both present
Availability:
Reference: PMID:11891286
Notes: CU428 transformed by pTTMN construct and cells selected with 10 mg/ml paromomycin. Partially replaced. Still has wt Metallothionein gene copies in mac as well as other alleles with neo coding.

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Cite this (TSC Cat# SD02600, RRID:TSC_SD02600)

Source Database: TSC, catalog # SD02600
Genetic Background: mpr1-1/mpr1-1 (ema1[3’neo3,HAc]/?EMA1; pm-r, VII)
Affected Genes: EMA1 (TTHERM_00088150)
Genomic Alteration: Macronucleus: C-terminal HA tag of EMA1 with neo3 in 3’flank
Availability:
Reference: PMID:18708581
Notes: They are not complete replacement From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01496, RRID:TSC_SD01496)

Source Database: TSC, catalog # SD01496
Genetic Background: wild type D (wild type D; wild type D, II)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD02599, RRID:TSC_SD02599)

Source Database: TSC, catalog # SD02599
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4] (drh1[∆?::neo4]/drh1[∆?::neo4]; pm-r, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 at 5’end of coding, some coding remains Macronucleus: neo4 at 5’ end of coding, some coding remains
Availability:
Reference:
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ C10-11F Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well. Needs Cd for pm resistance. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01999, RRID:TSC_SD01999)

Source Database: TSC, catalog # SD01999
Genetic Background: ∆HTB1/∆HTB1 pm-r with neo2 (pm-r, cy-s)
Affected Genes: HTB1 (TTHERM_00633360)
Genomic Alteration:
Availability:
Reference:
Notes: HTB1 complete KO strain. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01537, RRID:TSC_SD01537)

Source Database: TSC, catalog # SD01537
Genetic Background: gal1-1/gal1-1; tyr-14/tyr-14 (gal1-1; tyr-14; gal-r; tyr auxotroph, II)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:6950403
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD01631, RRID:TSC_SD01631)

Source Database: TSC, catalog # SD01631
Genetic Background: presumed phg-/phg- (presumed phg-; phg-, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:23195323, PMID:18658256, PMID:18311715
Notes: The Frankel lab could not obtain progeny from crosses using this stock. Its observations are consistent with the reported temperature-sensitive food-vacuoleless phenotype, but not consistent with the reported total loss of oral structures. This stock exhibits multiple unique morphogenetic phenotypes at 37.5o C.: see Frankel (2008: p. 1626, 1632). The Iowa stock is designated II8G-IA to distinguish it from the II8G stock, deposited by L. Rasmussen at the American Type Culture Collection. ATCC. Submitted by Joseph Frankel, University of Iowa

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Cite this (TSC Cat# SD02403, RRID:TSC_SD02403)

Source Database: TSC, catalog # SD02403
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (htax[K123R]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[K130R]/htaz[∆;;neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 knockout of HTAX, HTA1, HTAZ Macronucleus: mutant HTAX and HTAZ rescue of mac KO
Availability:
Reference: PMID:19822522
Notes: HTAX/HTAZ double mutant rescue; Rescue of h∆∆HTA∆HTZ1-F1B1 and B1C5 with Dryl's. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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