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on page 1 showing 20 out of 115 results from 1 sources

Cite this (TSC Cat# SD01951, RRID:TSC_SD01951)

Source Database: TSC, catalog # SD01951
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Availability:
Reference: PMID:16908532
Notes: non-star side of H3.3+GFP germline replacement heterokaryon. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02312, RRID:TSC_SD02312)

Source Database: TSC, catalog # SD02312
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: c-terminal HHT2 FLAG tagged
Availability:
Reference: PMID:9343391, PMID:16908532
Notes: Don’t know if complete replacement. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01864, RRID:TSC_SD01864)

Source Database: TSC, catalog # SD01864
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Availability:
Reference: PMID:16908532, PMID:15701804
Notes: homozygous, Neo cassette knockout before we knew there was HHT4 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02373, RRID:TSC_SD02373)

Source Database: TSC, catalog # SD02373
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF1 Macronucleus: KO of HHT1, HHF1;and for HHT2 have mutations S10A and S28A
Availability:
Reference: PMID:16908532, PMID:10199406
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #1. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01947, RRID:TSC_SD01947)

Source Database: TSC, catalog # SD01947
Genetic Background: hht3[5’neo2,∆,::(HHT2,GFPc)]/hht3[5’neo2, ∆,(::HHT2,GFPc)] (?)
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 into HHT3 locus
Availability:
Reference: PMID:16908532
Notes: star side of H3.2+GFP germline replacement heterokaryon. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01860, RRID:TSC_SD01860)

Source Database: TSC, catalog # SD01860
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT1 + HHT3 (TTHERM_00570560 + TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT3
Availability:
Reference: PMID:16908532
Notes: homozygous, Neo cassette knockout. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02641, RRID:TSC_SD02641)

Source Database: TSC, catalog # SD02641
Genetic Background: hht4[∆::neo2]/hht4[∆::neo2]
Affected Genes: HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT4 is replaced by neo2
Availability:
Reference: PMID:16908532
Notes: Germline HHT4 KO heterokaryon HHT4 coding is replaced by neo2, non* side From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01780, RRID:TSC_SD01780)

Source Database: TSC, catalog # SD01780
Genetic Background: mpr1-1/mpr1-1 (MTT1?/mtt1[5’neo2, ∆, (::HHT3,GFPc)]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: HHT3 with GFP c-terminal tag replaces the MTT1 coding region
Availability:
Reference: PMID:16908532
Notes: Somatic replace MTT1 coding with H3.3+GFP, neo 2 cassette in 5’ flank. Not sure if complete replacement. Makes hv2 histone variant protein. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01960, RRID:TSC_SD01960)

Source Database: TSC, catalog # SD01960
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01318, RRID:TSC_SD01318)

Source Database: TSC, catalog # SD01318
Genetic Background: mpr1-1/mpr1-1 (hht3[∆::neo2]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: KO of HHT3 with neo2 cassette
Availability:
Reference: PMID:16908532, PMID:9343391
Notes: HHT3 complete knockout , neo cassete replaces coding region. From 1400 ug/ml pm well. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02038, RRID:TSC_SD02038)

Source Database: TSC, catalog # SD02038
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hhf1[∆::neo]/hhf1[∆::neo] (mpr1-1; mp-r, ?)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo into HHT1, HHF1, HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: These are intermediate germline KO heterokaryons. May be early batch and not tested for somatic rescue. Final germline KO strains are #4 and #7. Non star side. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02646, RRID:TSC_SD02646)

Source Database: TSC, catalog # SD02646
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:16908532, PMID:9343391
Notes: HA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02214, RRID:TSC_SD02214)

Source Database: TSC, catalog # SD02214
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1] (?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
Reference: PMID:16908532
Notes: Germline double KO heterokaryon of HHT3(by neo2 cassette) and HHT4(by blasticidin cassette). Non* side. Mates with 3N4B-KO-* From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02556, RRID:TSC_SD02556)

Source Database: TSC, catalog # SD02556
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1] (hht3[∆::neo2];hht4[∆::bsr1]; pm-r, bsr-r, ?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1 Macronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
Reference: PMID:16908532
Notes: Homozygous homokaryon progeny of crossing ∆3N4B heterokaryons, C1nbe mated with ∆3N4B-6 This strain was stated to be pm-r, but proved not to be when thawed. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01865, RRID:TSC_SD01865)

Source Database: TSC, catalog # SD01865
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Availability:
Reference: PMID:16908532, PMID:15701804
Notes: homozygous, Neo cassette knockout before we knew there was HHT4 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02579, RRID:TSC_SD02579)

Source Database: TSC, catalog # SD02579
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 with GFP tagged H3 chimera protein 3, H3(1-75) H3.3(76-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Availability:
Reference: PMID:16908532
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein 3, H3(1-75) H3.3(76-135), rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01376, RRID:TSC_SD01376)

Source Database: TSC, catalog # SD01376
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:9343391, PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

  • From Current Category

Cite this (TSC Cat# SD01373, RRID:TSC_SD01373)

Source Database: TSC, catalog # SD01373
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: hht2[3’ neo2,FLAGc]
Availability:
Reference: PMID:16908532, PMID:9343391
Notes: Somatically replace HHT2 gene with HHT2-Flag sequence, neo 2 cassette is inserted in the 3’ flanking region of HHT2. Unknown if replacement is complete. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02378, RRID:TSC_SD02378)

Source Database: TSC, catalog # SD02378
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1,HHT2, HHF2 Macronucleus: KO of HHT1, HHF2; and for HHT2 have mutation S28A
Availability:
Reference: PMID:16908532, PMID:10199406
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S28A#8. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

  • From Current Category

Cite this (TSC Cat# SD02314, RRID:TSC_SD02314)

Source Database: TSC, catalog # SD02314
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:9343391, PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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