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on page 1 showing 20 out of 115 results

Cite this (TSC Cat# SD02647, RRID:TSC_SD02647)

Source Database: TSC, catalog # SD02647
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[3’neo2, HAn]; pm-r, VII)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:16908532, PMID:9343391
Notes: HA-tagged hv2 transformant. Checked by western blot and immunofluorescent staining From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01378, RRID:TSC_SD01378)

Source Database: TSC, catalog # SD01378
Genetic Background: hht4[∆::neo2]/hht4[∆::neo2] (wt)
Affected Genes: HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT4 is replaced by neo2
Availability:
Reference: PMID:16908532
Notes: Germline HHT4 KO heterokaryon HHT4 coding is replaced by neo2, non * side From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02374, RRID:TSC_SD02374)

Source Database: TSC, catalog # SD02374
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1,HHT2, HHF1 Macronucleus: KO of HHT1, HHF1; and for HHT2 have mutations S10A and S28A
Availability:
Reference: PMID:10199406, PMID:16908532
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #1. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02039, RRID:TSC_SD02039)

Source Database: TSC, catalog # SD02039
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hhf1[∆::neo]/hhf1[∆::neo] (mpr1-1; mp-r, ?)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo into HHT1, HHF1, HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: These are intermediate germline KO heterokaryons. May be early batch and not tested for somatic rescue. Final germline KO strains are #4 and #7. Non-star side. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01374, RRID:TSC_SD01374)

Source Database: TSC, catalog # SD01374
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:9343391, PMID:16908532
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01869, RRID:TSC_SD01869)

Source Database: TSC, catalog # SD01869
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01854, RRID:TSC_SD01854)

Source Database: TSC, catalog # SD01854
Genetic Background: hht2[3’neo2,∆ (::HHT3,GFPc)]/hht2[3’neo2,∆,(::HHT2,GFPc)]
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT3 into HHT2 locus
Availability:
Reference: PMID:16908532
Notes: Homozygous heterokaryon, can be mated with g-H3.3+GFP/H3.2-6 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02638, RRID:TSC_SD02638)

Source Database: TSC, catalog # SD02638
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (HHF2HHT2-GFP; pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP H3.2 with wt HHF2
Availability:
Reference: PMID:16908532
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3.2 ) rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02582, RRID:TSC_SD02582)

Source Database: TSC, catalog # SD02582
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 with GFP tagged H3 chimera protein 6, H3(1-56) H3.3(57-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Availability:
Reference: PMID:16908532
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein 6, H3(1-56) H3.3(57-135), rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01949, RRID:TSC_SD01949)

Source Database: TSC, catalog # SD01949
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02376, RRID:TSC_SD02376)

Source Database: TSC, catalog # SD02376
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1,HHT2, HHF1 Macronucleus: KO of HHT1, HHF1;and for HHT2 have mutations S10A and S28A
Availability:
Reference: PMID:16908532, PMID:10199406
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #6-later after seq.transformant found a wrong nucleotide~25 bases upsteam of ATG, transformant seems normal. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02215, RRID:TSC_SD02215)

Source Database: TSC, catalog # SD02215
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1] (?)
Affected Genes: HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT3 KO with neo2, HHT4 KO with bsr1
Availability:
Reference: PMID:16908532
Notes: Germline double KO heterokaryon of HHT3(by neo2 cassette) and HHT4(by blasticidin cassette). Non* side. Mates with 3N4B-KO-* From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02639, RRID:TSC_SD02639)

Source Database: TSC, catalog # SD02639
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (HHF2HHT.3; pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP H3.3 with wt HHF2 into HHT2HHF2 loccus
Availability:
Reference: PMID:16908532
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.HHT.3 inyo HHT2 locus with HHF2. HHT3 wt is still there. can be mated with B2086. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01778, RRID:TSC_SD01778)

Source Database: TSC, catalog # SD01778
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP,∆HHT3,∆HHT4; pm-r,bsr-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2 Macronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Availability:
Reference: PMID:16908532
Notes: H3.2-GFP homozygous heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02791, RRID:TSC_SD02791)

Source Database: TSC, catalog # SD02791
Genetic Background: hht2[3’neo2,GFPc]/hht2[3’neo2,GFPc] (hht2[3’neo2,GFPc]; pm-r, ?)
Affected Genes: HHT1 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 Macronucleus: GFP tagged HHT2
Availability:
Reference: PMID:16908532
Notes: Round II progeny of crossing H3.2-GFP-3x-10 (box 72:7 and 6) heterokaryons. Selected by pm and homokaryon was obtained. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01375, RRID:TSC_SD01375)

Source Database: TSC, catalog # SD01375
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
Reference: PMID:16908532, PMID:9343391
Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

  • From Current Category

Cite this (TSC Cat# SD01956, RRID:TSC_SD01956)

Source Database: TSC, catalog # SD01956
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines. From the Gorovsky lab, University of Rochester. NOTE: The description received from the Gorovosky lab indicated that the line is 6mp resistant. However, the thawed cells do not show resistance.

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Cite this (TSC Cat# SD02527, RRID:TSC_SD02527)

Source Database: TSC, catalog # SD02527
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2];hht3[∆::neo2]/hht3[∆::neo2]; hht2[3’neo2,S10E]/ hht2[3’neo2,S10E]; mpr1-1/mpr1-1 (hht1[∆::neo2]]; hht3[∆::neo2]; hht2[3’neo2,S10E]; pm-r, mp-r, ?)
Affected Genes: HHT1 (TTHERM_00570560) + HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of HHT1.HHT3,HHT2 has S10E mutaion and neo2 in 3’ flanking region Macronucleus: Neo KO of HHT1.HHT3,HHT2 has S10E mutaion and neo2 in 3’ flanking region
Availability:
Reference: PMID:16908532, PMID:15701804
Notes: H3 with S10E mutation in a background with no wildtype H3 present. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

  • From Current Category

Cite this (TSC Cat# SD02037, RRID:TSC_SD02037)

Source Database: TSC, catalog # SD02037
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hhf1[∆::neo]/hhf1[∆::neo] (mpr1-1; mp-r, ?)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo into HHT1, HHF1, HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: These are intermediate germline KO heterokaryons. May be early batch and not tested for somatic rescue. Final germline KO strains are #4 and #7. Star side. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

  • From Current Category

Cite this (TSC Cat# SD02614, RRID:TSC_SD02614)

Source Database: TSC, catalog # SD02614
Genetic Background: hht3[∆::neo2]/hht3[∆::neo2];hht4[∆::bsr1]/hht4[∆::bsr1];hht2[GFPc]/hht2[GFPc] (HHT2-GFP, ∆HHT3, ∆HHT4; pm-r,bsr-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHT3 (TTHERM_00016170), HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2 Macronucleus: Neo KO of HHT3, Bsr KO of HHT4, GFP tagged HHT2
Availability:
Reference: PMID:16908532
Notes: H3.2-GFP heterokaryon is crossed with ∆3N4B heterokaryon, the heterozygotes then go through Round I and the * side and non* side are then mated to obtain the homozygous homokaryons. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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