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on page 1 showing 20 out of 2,243 results from 1 sources

Cite this (TSC Cat# SD02828, RRID:TSC_SD02828)

Source Database: TSC, catalog # SD02828
Genetic Background: btu1-1[K350M]/ btu1-1[K350M] (BTU1 locus replaced by far 5’BTU1 5’--neo3---GFP coding--BTU1 3’; pm-r with Cd+2; tax-r, ?)
Affected Genes:
Genomic Alteration: Macronucleus: BTU1 locus replaced by far 5’BTU1 5’--neo3---GFP coding--BTU1 3’
Availability:
Reference: PMID:17194754
Notes: Complete somatic transformant of CU522 cells. Construct was BTU1 5’-Neo3---GFP coding- BTU1 3”. BTU1 promoter was deleted. Control for CDC2-GFP fusions - see paper. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02680, RRID:TSC_SD02680)

Source Database: TSC, catalog # SD02680
Genetic Background: htay[∆123-388::neo3]/htay[∆123-388::neo3] (HTAY/HTAY, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Micronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene
Availability:
Reference: PMID:22426537
Notes: Knockout Heterokaryon of HTAY gene. Different mating type from 6-2, 6-3, 6-6, 6-12. Same as 3-2-5 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01825, RRID:TSC_SD01825)

Source Database: TSC, catalog # SD01825
Genetic Background: chx1-1/chx1-1 (giw1[3’neo3,V5c]; p115:: p115-V5-neo3, pm-r V5 tagged GIW1p, VI)
Affected Genes: p115, GIWI (TTHERM_01276320 )
Genomic Alteration: Macronucleus: All GIW1 genes replaced by c-terminal V5tagged version with neo3 in 3’ flanking region
Availability:
Reference: PMID:20211138
Notes: All p115 copy in Mac was replaced with p115-V5-neo3 construct in CU427. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01367, RRID:TSC_SD01367)

Source Database: TSC, catalog # SD01367
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2]; mpr1-1/mpr1-1 (GTU1, mpr1-1; mp-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 replaces coding of GTU1
Availability:
Reference: PMID:12356864
Notes: GTU germline heterokaryon, progeny from CU428 x B2086 transformed with p∆GNII construct, can mate with CU427, CU428. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02474, RRID:TSC_SD02474)

Source Database: TSC, catalog # SD02474
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, K286E), HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
Availability:
Reference: PMID:12356864, PMID:16344310
Notes: pK268EHA rescued GTUKO 5x6 progeny having mutation @ K268E in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce. Not single celled yet. They were ~2 weeks old. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02059, RRID:TSC_SD02059)

Source Database: TSC, catalog # SD02059
Genetic Background: htax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2 (mp-r?, II)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 replaces coding of HTAX, HTA1, HTB1,HTB2
Availability:
Reference: PMID:19822522
Notes: HTX1/HTB1 quadruple KO heterokaryons;From mating of h∆∆HTA-F14A and h∆∆HTB-B3D3; made with B*VII. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02698, RRID:TSC_SD02698)

Source Database: TSC, catalog # SD02698
Genetic Background: (mlh1[243::neo2]; pm-r, ?)
Affected Genes: MLH1 (TTHERM_00471820)
Genomic Alteration: Macronucleus: Neo2 cassette inserted into coding of MLH1
Availability:
Reference: PMID:7606784
Notes: Transformation of CU428xB2086, complete knockout of mic histone linker gene with neo2 cassette inserted into Stu1 site. Paromomycin res. to 2 mg/ml Cell lines LH3 and LH6 can mate with each other. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01440, RRID:TSC_SD01440)

Source Database: TSC, catalog # SD01440
Genetic Background: hpo1-2/hpo1-2 (hpo1-2, II)
Affected Genes: HPO1 (hypoangular)
Genomic Alteration:
Availability:
Reference: PMID:8253268
Notes: A complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. The phenotype is more strongly expressed at 28 degrees C and less strongly at 39 degrees than it is in hpo1-1 (IA393). Submitted by Joseph Frankel, University of Iowa

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Cite this (TSC Cat# SD02756, RRID:TSC_SD02756)

Source Database: TSC, catalog # SD02756
Genetic Background: twi1[∆::neo3]/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, homozygous Macronucleus: TWI1 knockout but assorted to pm-s
Availability:
Reference: PMID:12297043
Notes: Homozygous homokaryon of TWI1 KO. neo3 was deleted and assorted to pm-s, can mate with c∆TWI1 WG7-pms-1-2. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01277, RRID:TSC_SD01277)

Source Database: TSC, catalog # SD01277
Genetic Background: CHX1[C3]/CHX1[C3]; MPR[C3]/MPR[C3]; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3];MPR[C3]; PMR1[C3]; MAT3; cy-s, mp-s, pm-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8601476, PMID:8725229
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD01951, RRID:TSC_SD01951)

Source Database: TSC, catalog # SD01951
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Availability:
Reference: PMID:16908532
Notes: non-star side of H3.3+GFP germline replacement heterokaryon. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD03023, RRID:TSC_SD03023)

Source Database: TSC, catalog # SD03023
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 7/24/2009 from the pond designated Bald Hill Trail Hd Wet in NH (latitude 43.12, longitude -72.21) It has a micronucleus.

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Cite this (TSC Cat# SD01591, RRID:TSC_SD01591)

Source Database: TSC, catalog # SD01591
Genetic Background: cdaJ-1/cdaJ-1 (cdaJ-1; cdaJ, VII)
Affected Genes: CDAJ (cell division arrest J)
Genomic Alteration:
Availability:
Reference:
Notes: Temperature-sensitive fission arrest with a high degree of disorganization of ciliary rows.Not extensively studied. Submitted by Joseph Frankel, University of Iowa

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Cite this (TSC Cat# SD02385, RRID:TSC_SD02385)

Source Database: TSC, catalog # SD02385
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ S134A]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
Availability:
Reference: PMID:11430834, PMID:17242195
Notes: It is somatic rescue of strain of HTA1,HTA2 double knockout by HTA1 S134A mutation. Same mating type as 1-1(3) From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02312, RRID:TSC_SD02312)

Source Database: TSC, catalog # SD02312
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: c-terminal HHT2 FLAG tagged
Availability:
Reference: PMID:9343391, PMID:16908532
Notes: Don’t know if complete replacement. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01695, RRID:TSC_SD01695)

Source Database: TSC, catalog # SD01695
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 5/31/2008 from the body of water designated Millstone Cr 3 in Sigel, PA (lat. 41 21.820, long. -79 04.164). Has a micronucleus. THIS STRAIN IS EXTINCT AND IS LISTED FOR HISTORICAL PURPOSES.

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Cite this (TSC Cat# SD01968, RRID:TSC_SD01968)

Source Database: TSC, catalog # SD01968
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:10880474
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD01864, RRID:TSC_SD01864)

Source Database: TSC, catalog # SD01864
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Availability:
Reference: PMID:16908532, PMID:15701804
Notes: homozygous, Neo cassette knockout before we knew there was HHT4 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02373, RRID:TSC_SD02373)

Source Database: TSC, catalog # SD02373
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF1 Macronucleus: KO of HHT1, HHF1;and for HHT2 have mutations S10A and S28A
Availability:
Reference: PMID:16908532, PMID:10199406
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #1. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01286, RRID:TSC_SD01286)

Source Database: TSC, catalog # SD01286
Genetic Background: chx?/chx?; pmr1-1/pmr1-1; MAT3/MAT3 (chx?;pmr1-1; MAT3; cy-?, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8725229, PMID:8601476
Notes: Resistance to 6-methylpurine was not scored for these panel members. Submitted by Eduardo Orias, University of California, Santa Barbara

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