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on page 1 showing 20 out of 2,241 results from 1 sources

Cite this (TSC Cat# SD01299, RRID:TSC_SD01299)

Source Database: TSC, catalog # SD01299
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; MAT2/MAT2 (chx1-1;pmr1-1; MAT2; cy-r, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8601476, PMID:8725229
Notes: Resistance to 6-methylpurine was not scored for these panel members. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD03281, RRID:TSC_SD03281)

Source Database: TSC, catalog # SD03281
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-126; Basal body disorganization and stability defects (TS), ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-126
Availability:
Reference: PMID:21562224
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 R126A, E127A, E130A. From the Winey Lab at the University of Colorado Boulder.

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Cite this (TSC Cat# SD00253, RRID:TSC_SD00253)

Source Database: TSC, catalog # SD00253
Genetic Background: Mpr?/Mpr?; Nulli 1,5 (MPR1; cy-r, mp-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes:

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Cite this (TSC Cat# SD01830, RRID:TSC_SD01830)

Source Database: TSC, catalog # SD01830
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445A]; mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445A or neo replacing coding of some ATU1 genes
Availability:
Reference: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pA445 derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E445A. The strain was supposed to be pm-r, but the thawed line is pm-s. From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD02781, RRID:TSC_SD02781)

Source Database: TSC, catalog # SD02781
Genetic Background: chx1-1/chx1-1 (twi1[3’neo3, FLAG-HA]; pm-r, VI)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Availability:
Reference: PMID:12297043
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU427 background. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02013, RRID:TSC_SD02013)

Source Database: TSC, catalog # SD02013
Genetic Background: btu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (assorted to pm-s so BTU1 assorted to bsr-s so BTU2; mp-r, VII)
Affected Genes: BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: BTU1 coding replaced with neo1 cassette; BTU2 is blasticidin KO. Macronucleus: assorted to WT or lost resistance cassettes
Availability:
Reference: PMID:10831613
Notes: Obtained from Jacek Gaertig, Jianming single celled. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO. Mates with DB2Apm-1.-2,-3 for heterokaryon rescue. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02624, RRID:TSC_SD02624)

Source Database: TSC, catalog # SD02624
Genetic Background: hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (mpr1-1; mp-r, II)
Affected Genes: HHT2 (TTHERM_00189180) + HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 replaces the HHF2 gene and HHT2 gene and the sequence between them
Availability:
Reference: PMID:15701804
Notes: The germline knockout heterokaryon of HHT2/HHF2 and the intergenic region has neo cassette completely replacing coding region. 3 independent lines #9, #10, #11. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01922, RRID:TSC_SD01922)

Source Database: TSC, catalog # SD01922
Genetic Background: MPR1/mpr1-1 (btu1[∆, H4-neo]; pm-r, IV)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: histone H4 promoter sequence then neo coding to replace the BTU2 coding region.
Availability:
Reference:
Notes: Complete disruption of BTU2 by an H4-neo cassette. CU428xB2086 electroporation transformation with histone H4 promoter sequence then neo coding to replace the BTU2 coding region. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02496, RRID:TSC_SD02496)

Source Database: TSC, catalog # SD02496
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo4,∆,HAHAn(::TCD2)]; pm-r, VII)
Affected Genes: HPL7 (TTHERM_00551070)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD2, neo4 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Availability:
Reference:
Notes: HA2-TCD2 overexpession from MTT1 locus, neo 4 upstream. HA-Tcd2p localizes to micronuclei in Growing and conjugation stage. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01816, RRID:TSC_SD01816)

Source Database: TSC, catalog # SD01816
Genetic Background: twi1[∆::neo3]/twi1[∆::neo3] (twi1[∆::neo3]; ∆TWI1, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, homozygous Macronucleus: TWI1 knockout
Availability:
Reference: PMID:12297043
Notes: Homozygous homokaryon of TWI1 KO. can mate with c∆TWI1 -WG4 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01719, RRID:TSC_SD01719)

Source Database: TSC, catalog # SD01719
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 7/25/2009 from the body of water designated Childs Bog in Harrisville, NH (lat. 42 57.763, long. -72 07.451). No micronucleus present.

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Cite this (TSC Cat# SD03206, RRID:TSC_SD03206)

Source Database: TSC, catalog # SD03206
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 9/4/2010 from the pond designated Peck's Pond in PA (latitude 41.28, longitude -75.08) It has a micronucleus.

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Cite this (TSC Cat# SD01960, RRID:TSC_SD01960)

Source Database: TSC, catalog # SD01960
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02564, RRID:TSC_SD02564)

Source Database: TSC, catalog # SD02564
Genetic Background: mpr1-1/mpr1-1 (mtt1-?[5’neo2,∆::BLT1, HAc], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’flanking region
Availability:
Reference:
Notes: CU428 somatic replacement with BLT-HA tag in the MTT1 locus, has Neo2 in 5’ flanking region of MTT1. Wild type BLT1 gene still present. This is a partial replacement. Cannot single-cell, only some cells express the HA taggedBLT when induced with Cd. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01686, RRID:TSC_SD01686)

Source Database: TSC, catalog # SD01686
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 8/16/2007 from the body of water designated Seney NWR 1 in Germfask, MI (lat. 46 16.68, long. -85 57.21). No micronucleus present.

  • From Current Category

Cite this (TSC Cat# SD01192, RRID:TSC_SD01192)

Source Database: TSC, catalog # SD01192
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes:

  • From Current Category

Cite this (TSC Cat# SD01825, RRID:TSC_SD01825)

Source Database: TSC, catalog # SD01825
Genetic Background: chx1-1/chx1-1 (giw1[3’neo3,V5c]; p115:: p115-V5-neo3, pm-r V5 tagged GIW1p, VI)
Affected Genes: p115, GIWI (TTHERM_01276320 )
Genomic Alteration: Macronucleus: All GIW1 genes replaced by c-terminal V5tagged version with neo3 in 3’ flanking region
Availability:
Reference: PMID:20211138
Notes: All p115 copy in Mac was replaced with p115-V5-neo3 construct in CU427. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02474, RRID:TSC_SD02474)

Source Database: TSC, catalog # SD02474
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, K286E), HAc]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
Availability:
Reference: PMID:12356864, PMID:16344310
Notes: pK268EHA rescued GTUKO 5x6 progeny having mutation @ K268E in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce. Not single celled yet. They were ~2 weeks old. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD00404, RRID:TSC_SD00404)

Source Database: TSC, catalog # SD00404
Genetic Background: Uni 3 [B or C3] (cnj3, pm-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes:

  • From Current Category

Cite this (TSC Cat# SD02064, RRID:TSC_SD02064)

Source Database: TSC, catalog # SD02064
Genetic Background: N terminal HA tag in TWI 11 with neo3 (?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration:
Availability:
Reference:
Notes: Homozygous heterokaryon HA -Twi 11 Mate with homozygous heterokaryon HA-Twi11 3-A7 From the Gorovsky lab, University of Rochester.

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