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on page 1 showing 20 out of 2,241 results

Cite this (TSC Cat# SD03080, RRID:TSC_SD03080)

Source Database: TSC, catalog # SD03080
Genetic Background: (?)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 6/30/2006 from the pond designated SG69-1 in PA (latitude 41.62, longitude -79.92) No micronucleus present. Did not mate.

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Cite this (TSC Cat# SD02872, RRID:TSC_SD02872)

Source Database: TSC, catalog # SD02872
Genetic Background: (hta1[∆::neo2], ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Macronucleus: neo2 into HTA1
Availability:
Reference: PMID:8754831, PMID:8760889
Notes: CET of CU428 x B2086 with plamid XL201, HTA1 with neo2 cassette replacing coding From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02373, RRID:TSC_SD02373)

Source Database: TSC, catalog # SD02373
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF1 Macronucleus: KO of HHT1, HHF1;and for HHT2 have mutations S10A and S28A
Availability:
Reference: PMID:16908532, PMID:10199406
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #1. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01286, RRID:TSC_SD01286)

Source Database: TSC, catalog # SD01286
Genetic Background: chx?/chx?; pmr1-1/pmr1-1; MAT3/MAT3 (chx?;pmr1-1; MAT3; cy-?, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8725229, PMID:8601476
Notes: Resistance to 6-methylpurine was not scored for these panel members. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD01298, RRID:TSC_SD01298)

Source Database: TSC, catalog # SD01298
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];pmr1-1; MAT2; cy-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8601476, PMID:8725229
Notes: Resistance to 6-methylpurine was not scored for these panel members. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD01324, RRID:TSC_SD01324)

Source Database: TSC, catalog # SD01324
Genetic Background: (pdd2[∆?::neo2], ?)
Affected Genes: PDD2 (TTHERM_00283530)
Genomic Alteration: Macronucleus: PDD2 with neo2 replacing the 5’ part of coding
Availability:
Reference: PMID:10805754, PMID:10557212
Notes: Progeny of B2086XCU428 transformation. Somatic KO of PDD2 gene. Mates with CU427, CU428, ∆PDD2-som-BC1, BC3 From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01279, RRID:TSC_SD01279)

Source Database: TSC, catalog # SD01279
Genetic Background: chx1-1/chx1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8725229, PMID:8601476
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, pm-s, mat3. Resistance to 6-methylpurine was not scored for these panel members. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD01978, RRID:TSC_SD01978)

Source Database: TSC, catalog # SD01978
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT3; cy-r, I)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:10880474
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous. Submitted by Eduardo Orias, University of California, Santa Barbara

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Cite this (TSC Cat# SD02012, RRID:TSC_SD02012)

Source Database: TSC, catalog # SD02012
Genetic Background: btu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (assorted to pm-s so BTU1 assorted to bsr-s so BTU2; mp-r, ?)
Affected Genes: BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: BTU1 coding replaced with neo1 cassette; BTU2 is blasticidin KO. Macronucleus: assorted to WT or lost resistance cassettes
Availability:
Reference: PMID:10831613
Notes: Obtained from Jacek Gaertig, Jianming single celled. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO. Mates with DB6Bpm-1.-2,-3 for heterokaryon rescue. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01914, RRID:TSC_SD01914)

Source Database: TSC, catalog # SD01914
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E433D,E435D]; pm-r, ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo knockout of btu2 with bsr Macronucleus: BTU1 has only (E431,433,435D)) no WT BTU2 is KO
Availability:
Reference: PMID:10831613, PMID:11864572
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431,433,435D). From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01801, RRID:TSC_SD01801)

Source Database: TSC, catalog # SD01801
Genetic Background: dcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?::neo3]/dcl1[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), DCL1 (TTHERM_00284230)
Genomic Alteration: Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Availability:
Reference: PMID:16738316, PMID:15598983
Notes: ∆DCL1 homokaryon transformed with Bsr-MTT1-CNA1-GFP. CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac. Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD01947, RRID:TSC_SD01947)

Source Database: TSC, catalog # SD01947
Genetic Background: hht3[5’neo2,∆,::(HHT2,GFPc)]/hht3[5’neo2, ∆,(::HHT2,GFPc)] (?)
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 into HHT3 locus
Availability:
Reference: PMID:16908532
Notes: star side of H3.2+GFP germline replacement heterokaryon. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD03199, RRID:TSC_SD03199)

Source Database: TSC, catalog # SD03199
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: Collected by Paul Doerder on 7/24/2009 from the pond designated Ashuelot Pond in NH (latitude 43.15, longitude -72.15) No micronucleus present.

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Cite this (TSC Cat# SD01960, RRID:TSC_SD01960)

Source Database: TSC, catalog # SD01960
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
Reference: PMID:16908532
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02564, RRID:TSC_SD02564)

Source Database: TSC, catalog # SD02564
Genetic Background: mpr1-1/mpr1-1 (mtt1-?[5’neo2,∆::BLT1, HAc], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’flanking region
Availability:
Reference:
Notes: CU428 somatic replacement with BLT-HA tag in the MTT1 locus, has Neo2 in 5’ flanking region of MTT1. Wild type BLT1 gene still present. This is a partial replacement. Cannot single-cell, only some cells express the HA taggedBLT when induced with Cd. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02626, RRID:TSC_SD02626)

Source Database: TSC, catalog # SD02626
Genetic Background: htax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2 (mp-r?, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 replaces coding of HTAX,HTA1,HTB1,HTB2
Availability:
Reference: PMID:19822522
Notes: HTX1/HTB1 quadruple KO heterokaryons;From mating of h∆∆HTA-F14A and h∆∆HTB-B3D3; made with B*VII. Not mating types II/VI/VII. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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Cite this (TSC Cat# SD02589, RRID:TSC_SD02589)

Source Database: TSC, catalog # SD02589
Genetic Background: dcl1[∆?::neo3]; twi11[HAn]/dcl1[∆?::neo3]; twi11[HAn] (?)
Affected Genes: DCL1 (TTHERM_00284230), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: Neo 3 into DCL1 and HA tagged TWI11 into the TWI11 locus
Availability:
Reference:
Notes: This strain has DCL1 KO with neo3 and n-terminus HA-tagged Twi11 in the mic. Mates with ∆DCL1 HAT11 #27 From the Gorovsky lab, University of Rochester.

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Cite this (TSC Cat# SD01637, RRID:TSC_SD01637)

Source Database: TSC, catalog # SD01637
Genetic Background: rmm9[C3]/rmm9[C3] (mp-s, pm-s, rrm9, III)
Affected Genes:
Genomic Alteration:
Availability:
Reference: PMID:8070657
Notes: rmm = rDNA maturation or maintenance mutation, located in the rDNA Submitted by Eileen Hamilton, University of California, Santa Barbara

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Cite this (TSC Cat# SD00022, RRID:TSC_SD00022)

Source Database: TSC, catalog # SD00022
Genetic Background: (VI)
Affected Genes:
Genomic Alteration:
Availability:
Reference:
Notes: B strain star line. Lacks a genetically functional micronucleus.

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Cite this (TSC Cat# SD01825, RRID:TSC_SD01825)

Source Database: TSC, catalog # SD01825
Genetic Background: chx1-1/chx1-1 (giw1[3’neo3,V5c]; p115:: p115-V5-neo3, pm-r V5 tagged GIW1p, VI)
Affected Genes: p115, GIWI (TTHERM_01276320 )
Genomic Alteration: Macronucleus: All GIW1 genes replaced by c-terminal V5tagged version with neo3 in 3’ flanking region
Availability:
Reference: PMID:20211138
Notes: All p115 copy in Mac was replaced with p115-V5-neo3 construct in CU427. From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.

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