Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

  • Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Search

Type in a keyword to search

on page 1 showing 5 out of 5 results

Cite this (BCBC Cat# 1001, RRID:BCBC_1001)

Source Database: BCBC, catalog # 1001
Genetic Background: RMCE
Affected Genes: pancreas specific transcription factor, 1a  
Genomic Alteration: Ptf1atm1
Availability: Cell line is available
Reference:
Notes: Diabetes mellitus Modified allele of Ptf1a with a biotinylation Tag sequence attached to the amino terminus of Ptf1a.

  • From Current Category

Cite this (BCBC Cat# 244, RRID:BCBC_244)

Source Database: BCBC, catalog # 244
Genetic Background: RMCE
Affected Genes: pancreas specific transcription factor, 1a  
Genomic Alteration: Ptf1atm1
Availability: Cell line is available
Reference:
Notes: Diabetes mellitus CreER knock-in into Ptf1a locus replacing part of the exon1 at the translation initiator ATG position using RMCE method.

  • From Current Category

Cite this (BCBC Cat# 207, RRID:BCBC_207)

Source Database: BCBC, catalog # 207
Genetic Background: RMCE
Affected Genes: pancreas specific transcription factor, 1a  
Genomic Alteration: Ptf1atm1
Availability: Cell line is available
Reference:
Notes: Diabetes mellitus Mice heterozygous for the tTA replacement of the Ptf1a gene are viable, fertile, of normal size, and do not display any behavioral abnormalities. The replacement places the expression of tTAoff (Gossen & Bujard, PNAS 89:5547, 1992) under the control of the regulatory sequences of the endogenous Ptf1a gene. This mouse was initially designed to be mated to a mouse (see BCBC #M461) bearing a bicistronic Ptf1a-lacZ transgene driven by the tetracycline-regulatory element (TRE: 7 copies of tetO with the CMV minimal promoter). For offspring homozygous for the tTA allele of Ptf1a and hemi- or homozygous for the transgene, the production of PTF1a is due solely to tTA-activation of the transgene, which is repressed by administration of tetracycline/doxycycline. This strategy allows embryonic developmental arrest at desired stages or cessation of gene function in adult mice for the pancreas (similar to that for Pdx1 described in Holland et al., PNAS 99:12236, 2002) and also for the cerebellum, retina, dorsal spinal cord and possibly hypothalamus. This transgenic mouse may be useful in studies of pancreatic endocrine/exocrine development and function, diabetes, and certain defects of the CNS. This tissue-specific expresser of tTA can also be bred with strains bearing other TRE-transgenes for organ-specific conditional expression analyses. Nearly all of the Ptf1a transcription unit has been replaced by a tTAoff coding sequence with accessory translational and RNA-processing signals, as follows, from 5' to 3': the Ptf1a gene transcriptional start and 49-bp of its 5'UTR; the Xenopus laevis beta-globin 5'UTR with Kozak initiator codon; the tTA coding sequence; and a 990-bp rabbit beta-globin gene fragment encoding the last 14-bp of its second exon, the 573-bp second intron, and the 364-bp last exon including a 98-bp 3'UTR with the polyA addition signal and addition site, and 39-bp of 3' beta-globin gene flanking sequence. The Ptf1a locus was modified by recombination-mediated cassette exchange using the cassette exchange allele in Ptf1a-LCA ES cells (Burlison et al., submitted).

  • From Current Category

Cite this (BCBC Cat# 218, RRID:BCBC_218)

Source Database: BCBC, catalog # 218
Genetic Background: RMCE
Affected Genes: pancreas specific transcription factor, 1a  
Genomic Alteration: Ptf1atm1
Availability: Cell line is available
Reference:
Notes: Diabetes mellitus This mouse strain has a 2-bp site-directed mutation in the endogenous Ptf1a locus that changes codon 298 from tryptophan to alanine. The mutation disrupts the ability of the PTF1a protein to bind RBPJ. Mice heterozygous for the mutation are viable, fertile, of normal size, and do not display any behavioral abnormalities. Mice homozygous for the mutation phenocopy the homozygous Ptf1a-null. Newborn homozygotes are apancreatic, devoid of the dI4 and dILA GABAergic dorsal interneurons, have an incomplete cerebellum, and die shortly after birth.

  • From Current Category

Cite this (BCBC Cat# 236, RRID:BCBC_236)

Source Database: BCBC, catalog # 236
Genetic Background: RMCE
Affected Genes: pancreas specific transcription factor, 1a  
Genomic Alteration: Ptf1atm1
Availability: Cell line is available; Now found at MMRRC
Reference:
Notes: Diabetes mellitus Ptf1aYFP mice may be used to visualize yellow fluorescent protein (YFP) expression from the ptf1a allele. Ptf1a is expressedin pancreatic buds beginning at E9.5 andplays a vital role in the growth and lineage specification of pancreatic multipotent progenitor cells (MPCs). Ptf1a is also expressed in the neuronal precursors of the cerebellum, spinal cord, and retina where it also performs fate determining roles.

  • From Current Category

  1. RRID Portal Resources

    Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.

  2. Navigation

    You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.

  3. Logging in and Registering

    If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.

  4. Searching

    Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:

    1. Use quotes around phrases you want to match exactly
    2. You can manually AND and OR terms to change how we search between words
    3. You can add "-" to terms to make sure no results return with that term in them (ex. Cerebellum -CA1)
    4. You can add "+" to terms to require they be in the data
    5. Using autocomplete specifies which branch of our semantics you with to search and can help refine your search
  5. Save Your Search

    You can save any searches you perform for quick access to later from here.

  6. Query Expansion

    We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.

  7. Collections

    If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.

  8. Sources

    Here are the sources that were queried against in your search that you can investigate further.

  9. Categories

    Here are the categories present within RRID that you can filter your data on

  10. Subcategories

    Here are the subcategories present within this category that you can filter your data on

  11. Further Questions

    If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.

X