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on page 1 showing 20 out of 553 results

Cite this (WB Cat# VT3077, RRID:WB-STRAIN:VT3077)

Source Database: WB, catalog # VT3077
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00001842(her-1), WBGene00006331(sup-26)
Genomic Alteration: WBVar00089681(n695)|WBVar00089955(n1091)|WBVar00142982(e128)
Availability: live
Reference:
Notes: outcrossed: x0; other notes: Pick wild-type GFP+ to maintain. Heterozygotes are WT with GFP+ pharynx, and segregate Dpy GFP+ mIn1 homozygotes, and GFP- low viability non-Egl hermaphrodites. Viability of first generation nDf50 homozygotes (GFP-) segregated from balanced heterozygotes is rescued by maternal contribution of mir-35-41 from balancer. Second generation GFP- animals are low viability. Reference: McJunkin K, Ambros V. Genes Dev. 2017 Feb 15;31(4):422-437.

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Cite this (WB Cat# CGC43, RRID:WB-STRAIN:CGC43)

Source Database: WB, catalog # CGC43
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference:
Notes: mutagen: CRISPR/Cas9; outcrossed: x; other notes: umnIs32 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Hets are WT GFP+ and segregate WT GFP+, Unc-4 (GFP-) and paralysed DpyUnc GFP+ (mnC1 homozygotes). Maintain by picking WT GFP+. Derived by insertion of myo-2p::GFP transgene into mnC1 balancer in parental strain SP127 using CRISPR/Cas9.

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Cite this (WB Cat# SP433, RRID:WB-STRAIN:SP433)

Source Database: WB, catalog # SP433
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00003866(ooc-1), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00089120(mn250)|WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference: WBPaper00000715
Notes: mutagen: EMS; outcrossed: x2; other notes: Hets are WT and segregate WT, paralysed DpyUnc and Sterile Unc-4's. ooc-1 homozygotes lay fertilized eggs that do not hatch. Oocytes are not rescuable by fertilization with N2 sperm."|"Made_by: Gail Spanier

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Cite this (WB Cat# VC1249, RRID:WB-STRAIN:VC1249)

Source Database: WB, catalog # VC1249
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00006735(ula-1)
Genomic Alteration: WBVar00142982(e128)|WBVar00531887(ok1700)
Availability: live
Reference:
Notes: mutagen: EMS; outcrossed: x1; other notes: C26E6.8. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok1700 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GGGTTTCCGGGGATATCTAA. External right primer: CGTACTGCCTGCATGAGAAA. Internal left primer: ATGTCATGCCACAAGGAACA. Internal right primer: CATTCTTGTGAAACTCGCCA. Internal WT amplicon: 2184 bp. Deletion size: 930 bp. Deletion left flank: GAATGTTGCCGACTCCTGAGTATGTCCATC. Deletion right flank: GAACGTCGGAAACTCATAAGAATCGCCTGA."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.

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Cite this (WB Cat# SP752, RRID:WB-STRAIN:SP752)

Source Database: WB, catalog # SP752
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference: WBPaper00000715
Notes: mutagen: X-ray; outcrossed: x; other notes: Hets are WT and segregate WT, DpyUnc and kinker Uncs which arrest as early larvae. Maintain by picking WT."|"Made_by: Chris Sigurdson

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Cite this (WB Cat# JK3172, RRID:WB-STRAIN:JK3172)

Source Database: WB, catalog # JK3172
Genetic Background:
Affected Genes: WBGene00000377(cct-1), WBGene00001072(dpy-10), WBGene00003241(mig-5)
Genomic Alteration: WBVar00091579(ok280)|WBVar00142982(e128)
Availability: live
Reference:
Notes: mutagen: UV/TMP; outcrossed: x5; other notes: Made_by: OMRF & K Siegfried"|"T05C12.6, T05C12.7. Heterozygotes are WT and GFP+ in the pharynx. mIn1[mIs14 dpy-10(e128)] homozygotes are Dpy and GFP+ in the pharynx. Homozygous mig-5&cct-1(ok280) worms arrest at about L2/L3. Strong loss of function for cct-1 and weak for mig-5. mig-5 is a dishelved homologue. External left primer: CGGCCTAAACGTTGATTGTT. External right primer: CAGAGTGAGTCGTGAACCGA. Internal left primer: TAATCCTGAATCCGGACGAG. Internal right primer: TACGAGATTTCGGTCCCTTG. Internal WT amplicon: 3284 bp. Deletion size: 987 bp. Deletion left flank: GCTGCAGTCTGATGTGCATGGCGGCTCCAT. Deletion right flank: TTGGTGTTGTCTATGCTTCAAGAAAATTGA. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

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Cite this (WB Cat# CB2987, RRID:WB-STRAIN:CB2987)

Source Database: WB, catalog # CB2987
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00006315(sup-6), WBGene00006752(unc-13)
Genomic Alteration: WBVar00142982(e128)|WBVar00143104(e309)|WBVar00248703(st19)
Availability: live
Reference:
Notes: mutagen: EMS; outcrossed: x; other notes: Dominant suppressor of Unc. Dpy. sup-6 is recessive lethal. Heterozygotes are Dpy non-Unc. Pick Dpy non-Unc to maintain."|"Made_by: Brenner S

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Cite this (WB Cat# PS295, RRID:WB-STRAIN:PS295)

Source Database: WB, catalog # PS295
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00002299(let-23), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)|WBVar00248909(sy97)
Availability: live
Reference: WBPaper00001404
Notes: mutagen: EMS; outcrossed: x; other notes: Heterozgyotes are WT and segregate WT, DpyUncs and Vulvaless Unc-4s. sy97 is only 15% viable. Maintain by picking WT. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects."|"Made_by: Raffi Arioan

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Cite this (WB Cat# VC2694, RRID:WB-STRAIN:VC2694)

Source Database: WB, catalog # VC2694
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00006728(ubq-2), WBGene00014176(ZK1010.2)
Genomic Alteration: WBVar00093217(ok2028)|WBVar00142982(e128)
Availability: live
Reference:
Notes: mutagen: EMS; outcrossed: x1; other notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK1010.2, ZK1010.1. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok2028 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCCAATTCAGGTCGTTCC. External right primer: TCATATCGAATTCATCGGCA. Internal left primer: TCCAAATGTTTTCCCGAGAG. Internal right primer: CTGGACGCTTGTTCAGCATA. Internal WT amplicon: 2149 bp. Deletion size: 896 bp. Deletion left flank: TGAAGCAACTGGGCGTCTCTTCTTCATCTT. Deletion right flank: GATTTTTCTTTAGAGACTAGTTTCAAAGGT.

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Cite this (WB Cat# GR1032, RRID:WB-STRAIN:GR1032)

Source Database: WB, catalog # GR1032
Genetic Background:
Affected Genes: WBGene00000090(age-1), WBGene00001072(dpy-10), WBGene00006787(unc-52)
Genomic Alteration: WBVar00088893(mg44)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference: WBPaper00001942
Notes: mutagen: EMS; outcrossed: x6; other notes: Heterozygotes are WT and segregate WT and DpyUnc. age-1(mg44) homozygotes from heterozygous mothers are WT and segregate only dauers at all temperatures. mg44 pka daf-23(mg44)."|"Made_by: S. Gottlieb

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Cite this (WB Cat# AN170, RRID:WB-STRAIN:AN170)

Source Database: WB, catalog # AN170
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00006744(unc-4), WBGene00006787(unc-52), WBGene00016625(aff-1)
Genomic Alteration: WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)|WBVar00266475(ty4)
Availability: live
Reference:
Notes: mutagen: EMS; outcrossed: x5; other notes: Heterozygotes are WT and segregate WT, Unc-4 worms which are strong Egl, and paralyzed Dpy Uncs."|"Made_by: J Choi/A Newman

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Cite this (WB Cat# PS1410, RRID:WB-STRAIN:PS1410)

Source Database: WB, catalog # PS1410
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00002299(let-23), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)|WBVar00248889(sy15)
Availability: live
Reference: WBPaper00002170
Notes: mutagen: EMS; outcrossed: x; other notes: Heterozygotes are WT and segregate WT, DpyUncs and Unc larval lethals. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects."|"Made_by: Aroian/Lesa

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Cite this (WB Cat# CB2744, RRID:WB-STRAIN:CB2744)

Source Database: WB, catalog # CB2744
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00002994(lin-5)
Genomic Alteration: WBVar00142982(e128)|WBVar00143936(e1348)
Availability: live
Reference: WBPaper00000496
Notes: outcrossed: x; other notes: Heterozygotes are WT. Segregates Dpy. Segregates sterile Unc--Unc cannot back. Balanced well."|"Made_by: Sulston J

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Cite this (WB Cat# VC354, RRID:WB-STRAIN:VC354)

Source Database: WB, catalog # VC354
Genetic Background:
Affected Genes: WBGene00000046(acr-7), WBGene00001072(dpy-10)
Genomic Alteration: WBVar00091891(ok605)|WBVar00142982(e128)
Availability: live
Reference:
Notes: mutagen: UV/TMP; outcrossed: x1; other notes: T09A5.3. Heterozygotes are WT with semi-dominant GFP expression in pharynx. Segregates WT GFP, Dpy GFP mIn1 homozygotes and ok605 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.

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Cite this (WB Cat# MT4106, RRID:WB-STRAIN:MT4106)

Source Database: WB, catalog # MT4106
Genetic Background:
Affected Genes: WBGene00000548(clr-1), WBGene00001072(dpy-10)
Genomic Alteration: WBVar00142982(e128)|WBVar00144275(e1745)
Availability: live
Reference:
Notes: outcrossed: x; other notes: Made_by: M. Stern"|"Maintain at 15C.

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Cite this (WB Cat# VC614, RRID:WB-STRAIN:VC614)

Source Database: WB, catalog # VC614
Genetic Background:
Affected Genes: WBGene00000767(coq-8), WBGene00001072(dpy-10)
Genomic Alteration: WBVar00092118(ok840)|WBVar00142982(e128)
Availability: live
Reference:
Notes: mutagen: UV/TMP; outcrossed: x1; other notes: C35D10.4. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpy mT1 homozygotes, and ok840 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.

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Cite this (WB Cat# KK375, RRID:WB-STRAIN:KK375)

Source Database: WB, catalog # KK375
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00003197(mel-12), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00088019(it42)|WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference: WBPaper00001109
Notes: mutagen: EMS; outcrossed: x>2; other notes: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating.

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Cite this (WB Cat# KK34, RRID:WB-STRAIN:KK34)

Source Database: WB, catalog # KK34
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00003201(mel-16), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00000392(b298)|WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference: WBPaper00001109
Notes: mutagen: EMS; outcrossed: x>2; other notes: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT.

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Cite this (WB Cat# VC1300, RRID:WB-STRAIN:VC1300)

Source Database: WB, catalog # VC1300
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00009202(aptf-4)
Genomic Alteration: WBVar00142982(e128)|WBVar00145949(gk582)
Availability: live
Reference:
Notes: mutagen: UV/TMP; outcrossed: x1; other notes: F28C6.1. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP gk582 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: ATGATAAGACGTCCTTGCCG. External right primer: TGCCTCTGCATTGTTCTCAC. Internal left primer: TGTTTGCACTGTTCGACGTT. Internal right primer: GGCGGATTGATTCATATGCT. Internal WT amplicon: 2222 bp. Deletion size: 1146 bp. Deletion left flank: GCGGTTTTTAAAATAACGTGAATATTGCTT. Deletion right flank: GACAAACACTGAGCGAGAAAACGAATCAGA."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.

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Cite this (WB Cat# KK357, RRID:WB-STRAIN:KK357)

Source Database: WB, catalog # KK357
Genetic Background:
Affected Genes: WBGene00001072(dpy-10), WBGene00003187(mel-1), WBGene00006744(unc-4), WBGene00006787(unc-52)
Genomic Alteration: WBVar00088000(it19)|WBVar00142975(e120)|WBVar00142982(e128)|WBVar00143214(e444)
Availability: live
Reference: WBPaper00001109
Notes: mutagen: EMS; outcrossed: x>2; other notes: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect.

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