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on page 1 showing 24 out of 3,330,710 results

    pCoofy52

https://www.addgene.org/122006/

Cite This: RRID:Addgene_122006

Species:
Genetic insert:
Vector Backbone: pET
Reference: PMID:23410102
Comment: ccdB Survival cells are NOT suitable for the unmodified plasmid containing the ccdB gene. The plasmid is provided in the recommended DB3.1 strain. Please see supplemental file for LP1 and LP2 primers to use for SLIC cloning. A detailed protocol for using pCoofy plasmids is available at https://p4eu.org/wiki . Use one of the following linearization primer pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer:LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3'LP2 reverse vector primer: SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3'Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.


Cite this (IMSR Cat# EM:08125, RRID:IMSR_EM:08125)

Source Database: IMSR, catalog # EM:08125
Genetic Background:
Affected Genes: Atp5g2
Genomic Alteration: tm2a(EUCOMM)Hmgu
Availability: mouse cells
Reference:
Notes: gene symbol note: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9); mutant strain: targeted mutation 2a, Helmholtz Zentrum Muenchen GmbH


https://web.expasy.org/cellosaurus/CVCL_4I48

Cite this: (Coriell Cat# GM11062, RRID:CVCL_4I48)
Organism: Homo sapiens
Disease:
Category: Transformed cell line DT Created: 22-09-15; Last updated: 05-07-19; Version: 7
Comment: Population: Amish Caucasian. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). DT Created: 22-09-15; Last updated: 05-07-19; Version: 7


https://www.ncbi.nlm.nih.gov/biosample/SAMN08482537

Cite this: (IIDP, Cat# 834, RRID:SAMN08482537)
Organism: Homo sapiens
Disease: Diabetes
Category: Tissue: Pancreas
Comment: IIDP Submission for Human Islets; Provider: Camilo Ricordi,University of Miami, Miami, FL, 33136; Age: 55.00 Years; Submission Date: 2018-02-07T18:06:03.360; Status of tissue: live


http://antibodyregistry.org/AB_378731

Cite this (GeneTex Cat# GTX27614, RRID:AB_378731)
Comments: manufacturer recommendations: IgG; IgG ELISA; Immunohistochemistry; Immunohistochemistry - fixed; Western Blot; Other; For ELISA: Use at a dilution of 1:5,000 - 1:10,000. For IHC-P: Use at a dilution of 1:250 - 1:500. For WB: Use at a dilution of 1:500 - 1:1,000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. ELISA, IHC (Formalin-fixed paraffin-embedded sections), Western blot.
References:
Host Organism: goat
Clonality: polyclonal antibody
Target(s): Apo AI antibody


    SciPipe

Cite this (SciPipe, RRID:SCR_017086)

URL: http://scipipe.org

Resource Type: Resource, source code, workflow software, data processing software, software library, software application, software resource, software toolkit

Software tool for writing scientific workflows of shell commands that depend on each other in Go programming language. Workflow library for agile development of complex and dynamic bioinformatics pipelines. Used in bioinformatics and cheminformatics, but works for any problem involving pipelines of commandline applications.


https://www.addgene.org/16798/

Cite This: RRID:Addgene_16798
fzd1; frizzled
Species: Rattus norvegicus
Genetic insert: Rfz1
Vector Backbone: pCS2+
Reference:
Comment: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.Rfz1 from Henk Roelink. This sequence has a few differences from the published sequence. Sites listed in Rfz1 are not a complete list. MYC tag version made by Jeff Brown (2/98-3/98), inserted the epitope into the StyI site (1904). The MYC epitope has a BglII site in it. The 3'Sty site is destroyed by the tags, but the 5' site is intact. Epitope can be detected in PBS/Formaldehyde fixed tissue permeabilized with 0.2% Triton.The Myc tagged Rfz1 has been IP'd from a TBS/1% Triton buffer. Frizzled tends to ppt out of SDS sample buffer when boiled, so either warm samplesto ~70 degrees Celsius for a few minutes before loading samples or use a strong denaturing loading buffer and incubate the samples at room temp for half an hour before loading. The StyI site is in the 3rd extracellular loop of the protein.


Cite this (WB Cat# MY2282, RRID:WB-STRAIN:MY2282)

Source Database: WB, catalog # MY2282
Genetic Background:
Affected Genes:
Genomic Alteration:
Availability: live
Reference:
Notes: other notes: species identified with species specific primers


https://web.expasy.org/cellosaurus/CVCL_4I49

Cite this: (Coriell Cat# GM11063, RRID:CVCL_4I49)
Organism: Homo sapiens
Disease:
Category: Transformed cell line DT Created: 22-09-15; Last updated: 05-07-19; Version: 7
Comment: Population: Amish Caucasian. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). DT Created: 22-09-15; Last updated: 05-07-19; Version: 7


https://www.ncbi.nlm.nih.gov/biosample/SAMN08554602

Cite this: (IIDP, Cat# 8, RRID:SAMN08554602)
Organism: Homo sapiens
Disease: Diabetes
Category: Tissue: Pancreas
Comment: IIDP Submission for Human Islets; Provider: Camilo Ricordi,University of Miami, Miami, FL, 33136; Age: 61.00 Years; Submission Date: 2018-02-15T23:50:04.100; Status of tissue: live


http://antibodyregistry.org/AB_378789

Cite this (GeneTex Cat# GTX20685, RRID:AB_378789)
Comments: manufacturer recommendations: ELISA; ELISA. The usefulness of this product in other applications has not been determined., ELISA, For ELISA: Use at an assay dependent dilution. Not tested in other applications. Optimal dillutions/concentrations should be determined by the researcher.
References:
Host Organism: rat
Clonality: monoclonal antibody
Target(s): Aflatoxin [1C6] antibody


    FSL

Cite this (FSL, RRID:SCR_002823)

URL: http://www.fmrib.ox.ac.uk/fsl/

Resource Type: Resource, software resource, software toolkit, software library

Software library of image analysis and statistical tools for fMRI, MRI and DTI brain imaging data. Include registration, atlases, diffusion MRI tools for parameter reconstruction and probabilistic taractography, and viewer. Several brain atlases, integrated into FSLView and Featquery, allow viewing of structural and cytoarchitectonic standard space labels and probability maps for cortical and subcortical structures and white matter tracts. Includes Harvard-Oxford cortical and subcortical structural atlases, Julich histological atlas, JHU DTI-based white-matter atlases, Oxford thalamic connectivity atlas, Talairach atlas, MNI structural atlas, and Cerebellum atlas.


https://www.addgene.org/76720/

Cite This: RRID:Addgene_76720
MAPK9; NM_001135044.1
Species: Homo sapiens
Genetic insert: MAPK9
Vector Backbone: lentiGuide-Puro
Reference: PMID:26780180
Comment: The gRNA was designed to target the following Genbank reference sequence(s): NM_001135044.1,NM_002752.4,NM_139068.2,NM_139069.2,NM_139070.2. Note that this plasmid does NOT contain Cas9. It should be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9. Browse the full kinome gRNA library at http://www.addgene.org/pooled-library/broadgpp-human-kinome/ or visit http://www.broadinstitute.org/rnai/public/ for detailed protocols and information.


Cite this (IMSR Cat# TIGM:IST14439C3, RRID:IMSR_TIGM:IST14439C3)

Source Database: IMSR, catalog # TIGM:IST14439C3
Genetic Background:
Affected Genes: Mybl2
Genomic Alteration: Gt(IST14439C3)Tigm
Availability: mouse cells
Reference:
Notes: gene symbol note: myeloblastosis oncogene-like 2; unclassified: gene trap IST14439C3, Texas A&M Institute for Genomic Medicine


https://web.expasy.org/cellosaurus/CVCL_4I50

Cite this: (Coriell Cat# GM11064, RRID:CVCL_4I50)
Organism: Homo sapiens
Disease:
Category: Transformed cell line DT Created: 22-09-15; Last updated: 05-07-19; Version: 7
Comment: Population: Amish Caucasian. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). DT Created: 22-09-15; Last updated: 05-07-19; Version: 7


https://www.ncbi.nlm.nih.gov/biosample/SAMN08579888

Cite this: (IIDP, Cat# 18, RRID:SAMN08579888)
Organism: Homo sapiens
Disease: Diabetes
Category: Tissue: Pancreas
Comment: IIDP Submission for Human Islets; Provider: Fouad Kandeel,Southern California Islet Cell Resources Center, Duarte, CA, 91010; Age: 51.00 Years; Submission Date: 2018-02-21T16:15:03.793; Status of tissue: live


http://antibodyregistry.org/AB_378799

Cite this (GeneTex Cat# GTX26705, RRID:AB_378799)
Comments: manufacturer recommendations: Immunodiffusion; Other; Immunoprecipitation; Immunohistochemistry; Conjugation, ID, IHC-Fr, IHC-P, IP
References:
Host Organism: rabbit
Clonality: polyclonal antibody
Target(s): Rabbit Porcine IgG (H+L)



https://www.addgene.org/91916/

Cite This: RRID:Addgene_91916

Species: Other
Genetic insert: PpCas13a and guide expression cassette
Vector Backbone: pACYC184
Reference: PMID:28976959
Comment:


Cite this (IMSR Cat# TIGM:IST14005D10, RRID:IMSR_TIGM:IST14005D10)

Source Database: IMSR, catalog # TIGM:IST14005D10
Genetic Background:
Affected Genes: Pak4
Genomic Alteration: Gt(IST14005D10)Tigm
Availability: mouse cells
Reference:
Notes: gene symbol note: p21 (RAC1) activated kinase 4; unclassified: gene trap IST14005D10, Texas A&M Institute for Genomic Medicine


https://web.expasy.org/cellosaurus/CVCL_4I51

Cite this: (Coriell Cat# GM11065, RRID:CVCL_4I51)
Organism: Homo sapiens
Disease: Manic bipolar affective disorder
Category: Transformed cell line DT Created: 22-09-15; Last updated: 05-07-19; Version: 7
Comment: Population: Amish Caucasian. Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV). DT Created: 22-09-15; Last updated: 05-07-19; Version: 7


https://www.ncbi.nlm.nih.gov/biosample/SAMN08498330

Cite this: (IIDP, Cat# 849, RRID:SAMN08498330)
Organism: Homo sapiens
Disease: Diabetes
Category: Tissue: Pancreas
Comment: IIDP Submission for Human Islets; Provider: Fouad Kandeel,Southern California Islet Cell Resources Center, Duarte, CA, 91010; Age: 63.00 Years; Submission Date: 2018-02-08T08:56:22.143; Status of tissue: live


http://antibodyregistry.org/AB_261191

Cite this (Sigma-Aldrich Cat# P8083, RRID:AB_261191)
Comments: Vendor recommendations: IgG2a Other; Western Blot; immunoblotting: 1:2,000 using rat brain cytosol preparation microarray: suitable
References:
Host Organism: mouse
Clonality: monoclonal antibody
Target(s): Protein Kinase Cgamma antibody produced in mouse


Cite this (U.S. Department of Agriculture, RRID:SCR_011486)

URL: http://www.usda.gov/

Resource Type: Resource, government granting agency

A United States Federal Executive Department to develop and execute policy on farming, agriculture, and food. It aims to meet the needs of farmers and ranchers, promote agricultural trade and production, work to assure food safety, protect natural resources, foster rural communities and end hunger, in America and abroad. USDA also participates in overseas aid programs, by providing surplus foods to developing countries to support development programs with other organizations. (http://en.wikipedia.org/wiki/USDA)


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