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Organism Name
OP50(xu363)
RRID:WB-STRAIN:WBStrain00041970 RRID Copied  
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RRID:WB-STRAIN:WBStrain00041970
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Organism Information

URL: http://www.wormbase.org/db/get?name=WBStrain00041970

Proper Citation: RRID:WB-STRAIN:WBStrain00041970

Description: Escherichia coli with name E. coli [ura-, strR, rnc-, (delta)attB::FRT-lacI-lacUV5p-T7). from WB.

Species: Escherichia coli

Synonyms: E. coli [ura-, strR, rnc-, (delta)attB::FRT-lacI-lacUV5p-T7).

Notes: Bacteria. RNAi-compatible OP50 strain. Slow growing. When growing this strain, pick single colonies for liquid culture (at least 20 hrs) from a freshly streaked tetracycline LB plate. Do not include tetracycline in liquid culture, as Tet in liquid culture can have long lasting effects on worm lifespan. The authors recommend using standard PEG transformation method to make competent OP50(xu363) cells, but other ways to make competent cells will also likely work for OP50(xu363). Reference: Xiao R, et al. Cell Rep. 2015 May 19;11(7):1123-33.|"No longer available from the CGC"|"Non-elegans Strain"|"WBStrain provided so WBPaper00060797 paper added based on AFP_Strain data."|"WBStrain provided so WBPaper00061998 paper added based on AFP_Strain data."

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Data and Source Information

Source: WormBase (RRID:SCR_003098)
Description: Central data repository for nematode biology including complete genomic sequence, gene predictions and orthology assignments from range of related nematodes.Data concerning genetics, genomics and biology of C. elegans and related nematodes. Derived from initial ACeDB database of C. elegans genetic and sequence information, WormBase includes genomic, anatomical and functional information of C. elegans, other Caenorhabditis species and other nematodes. Maintains public FTP site where researchers can find many commonly requested files and datasets, WormBase software and prepackaged databases.
URL: http://www.wormbase.org

Source Database: WormBase (WB)