University of California at San Francisco Immunology Lab Core Facility (RRID:SCR_017906)Copy Citation Copied
URL: https://coreimmunology.ucsf.edu/services
Proper Citation: University of California at San Francisco Immunology Lab Core Facility (RRID:SCR_017906)
Description: Provides immunology assays, expert consultation, and education to UCSF and global research community. CIL specializes in developing immunology research tools, and initiating and stimulating innovative research projects to address emerging questions in immunologic diseases including HIV pathogenesis.Services include:Isolation of PBMC for staining, cell sorting, or viable cryopreservation;Isolation of mucosal mononuclear cells (MMC) from mucosal tissue biopsies;Immunomagnetic bead isolation of untouched cell populations for functional studies;Immunophenotyping of whole blood, PBMC, MMC and cerebrospinal fluid for: NK, B and T cells percentages, and their subsets, including maturation, trafficking, senescence, activation, in vivo proliferation and chemokine receptor expression;Flow cytometric functional assays, including NK cell function, antigen-specific CD4+ and CD8+ T cell cytokine expression, degranulation and proliferation;Evaluation of T cell signaling by phosflow using monoclonal antibodies specific for phosphorylated protein;Training and Support;Flow Cytometry Training Course;Three-hour lecture and lab course designed to cover basics of flow cytometry including:Fluorescence emission and detection,Fluidics,Operation,Panel design,Controls.
Abbreviations: CIL
Synonyms: Core Immunology Lab, University of California at San Francisco Core Immunology Lab
Resource Type: service resource, core facility, access service resource
Keywords: ABRF, USEDit, immunology assays, immunologic diseases
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