URL: http://www.addgene.org/99372
Proper Citation: RRID:Addgene_99372
Insert Name: dCas9-KRAB-T2A-Puro
Organism: synthetic; S. pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28757163
Vector Backbone Description: Backbone Size:9192; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: dCas9 sequence is from Addgene plasmid #61425 (generously shared by Feng Zhang lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014 KRAB sequence is from Addgene plasmid #50919 (generously shared by Rene Maehr, Scot Wolfe lab). "Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.", Kearns et al. 2014
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org