URL: http://www.addgene.org/99372
Proper Citation: RRID:Addgene_99372
Insert Name: dCas9-KRAB-T2A-Puro
Organism: synthetic; S. pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28757163
Vector Backbone Description: Backbone Size:9192; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: dCas9 sequence is from Addgene plasmid #61425 (generously shared by Feng Zhang lab). "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.", Konermann et al.. 2014 KRAB sequence is from Addgene plasmid #50919 (generously shared by Rene Maehr, Scot Wolfe lab). "Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells.", Kearns et al. 2014
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Source: Addgene