URL: http://www.addgene.org/99154
Proper Citation: RRID:Addgene_99154
Insert Name: Cas9
Organism: Synthetic
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:10000; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: This LentiCRISPRv2 variant was produced by restriction digest and multi-fragment InFusion (Clontech) assembly. It has been validated for cutting efficiency following transduction and FACS-sorting for mCherry. Suitable for single- and multi-colour guided CRISPR editing in stem cells and cell lines. sgRNA of choice can be cloned in using BsmBI digest. Follow original LentiCRISPRv2 cloning protocol.
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Source: Addgene