URL: http://www.addgene.org/97411
Proper Citation: RRID:Addgene_97411
Insert Name: tTA
Organism: E. coli, herpes simplex virus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26655910
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6329; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The thy1S promoter used in this vector was originally provided by Dr. Kawaski in Kanazawa university. The reference for the original work is "Ako et al (2011) Simultaneous visualization of multiple neuronal properties with single-cell resolution in the living rodent brain. Mol Cell Neurosci 48:246–257." We shortened their sequence to accommodate in the AAV vector. It was originally used to sparsely label the marmoset neurons (ref: Sadakane et al. In Vivo Two-Photon Imaging of Dendritic Spines in Marmoset Neocortex(1,2,3). eNeuro. 2015 Sep17;2(4).). For two-photon calcium imaging, we used the vector at high concentration, at which condition, there seems to be low cell type specificity.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
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