URL: http://www.addgene.org/9044
Proper Citation: RRID:Addgene_9044
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:6500; Vector Backbone:modified pMSCV 2.2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Excised IRES hGFP-3M from pCITE IRES hGFP-3M with EcoRI/SalI digest. Ligated into MSCV2.2 following EcorI/SalI digest of vector. Lost puro resistance gene (pac) in process. Luk Van Parijs et al., Immunity 11(3): 281. Also see Addgene Plasmid #12282, pMIG-w, which is a variant of pMIG containing a WRE element. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold. Please see plasmid 20672: MSCV-IRES-GFP for sequence reference.
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Source: Addgene