URL: http://www.addgene.org/89463
Proper Citation: RRID:Addgene_89463
Insert Name: DeAct-SpvB
Organism: Salmonella enterica
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28394337
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3383; Vector Backbone:pTRE3G-luciferase; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Uses Clontech 3rd generation Tet-responsive promoter; also requires Tet-On 3G transactivator protein. E. coli dihydrofolate reductase destabilization domain (DHFRdd) with R12Y, G67S, Y100I mutations was a kind gift of Tom Wandless (Stanford): Iwamoto, M., Björklund, T., Lundberg, C., Kirik, D. & Wandless, T. J. A general chemical method to regulate protein stability in the mammalian central nervous system. Chem. Biol. 17, 981–988 (2010).
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No rating or validation information has been found for pTetON-DHFRdd-SpvB; CMV-mCherry.
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Source: Addgene