URL: http://www.addgene.org/8842
Proper Citation: RRID:Addgene_8842
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8635750
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid expression vector enables a protein of interest to be produced with a biotin acceptor peptide (BAP) fused directly to its N-terminus, or, alternatively, as a fusion to the C-terminus of E. coli maltose-binding protein with the BAP on its N-terminus. pDW363 also overproduces biotin holoenzyme synthetase (BirA) to ensure efficient biotinylation of BAP-tagged proteins in vivo. Add biotin to the medium (50 mM) when cultivating cells that contain pDW363 or it derivatives.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org