URL: http://www.addgene.org/8827
Proper Citation: RRID:Addgene_8827
Insert Name: TEV protease, S219V mutant
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:11809930
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMal-C2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Bacterial strain: E.coli BL21(DE3)-RIL (Stratagene). pRK793 overproduces the catalytic domain of tobacco etch virus (TEV) protease in the form of an MBP fusion protein that cleaves itself in vivo to yield a TEV protease catalytic domain with an N-terminal His-tag and a C-terminal polyarginine tag.
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Source: Addgene