URL: http://www.addgene.org/87377
Proper Citation: RRID:Addgene_87377
Insert Name: GFP dropout part
Bacterial Resistance: Ampicillin
Defining Citation: PMID:28230977
Vector Backbone Description: Backbone Marker:MoBioTec; Vector Backbone:pHT01; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: pCG004 is identical to pHT01 except for two changes. Firstly an unwanted backbone BsaI site with the AmpR cassette was removed. Secondly, the multiple-cloning site of pHT01 was replaced with a BsaI dropout part. This part consists of a constitutive GFP mut3b-expression cassette (using the Pveg promoter, spoVG RBS and rrnB terminator) flanked by BsaI restriction sites. During assembly of ORF parts into pCG004, the GFP-expression cassette is removed and the full-length ORF inserted downstream of the RBS and upstream of the terminator.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org