URL: http://www.addgene.org/87360
Proper Citation: RRID:Addgene_87360
Insert Name: Cas9-2A-eGFP
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30795624
Vector Backbone Description: Backbone Marker:Feng Zhang lab; Backbone Size:16733; Vector Backbone:lentiCRISPR v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Doxycycline inducible; eGFP reporter; Bacterial Resistance:Ampicillin
Comments: Clone sgRNAs into TLCV2 using the same protocol provided with lentiCRISPR v2 (Plasmid #52961, protocol listed under the RESOURCE INFORMATION section). After you have inserted your sgRNA, you should sequence this region with one of the following primers: hU6-F (5'-GAGGGCCTATTTCCCATGATT-3') or LKO.1 5’(5'- GACTATCATATGCTTACCGT-3').
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Source: Addgene