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Plasmid Name
RRID:Addgene_86973 RRID Copied  
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RRID:Addgene_86973
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Plasmid Information

URL: http://www.addgene.org/86973

Proper Citation: RRID:Addgene_86973

Insert Name: protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)

Organism: Staphylococcus aureus

Bacterial Resistance: Kanamycin

Defining Citation: PMID:15469830

Vector Backbone Description: Backbone Size:2661; Vector Backbone:pK19; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Comments: This plasmid expresses a pA-MN fusion protein in E. coli. The MN coding sequence was cloned into pK19SEVII (Giraud-Panis et al., 1995). The pA segment contains 2 IgG binding domains of staphylococcal protein A and was fused to MN with a linker sequence of DDDKEF.

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Data and Source Information

Source: Addgene