URL: http://www.addgene.org/86973
Proper Citation: RRID:Addgene_86973
Insert Name: protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
Organism: Staphylococcus aureus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:15469830
Vector Backbone Description: Backbone Size:2661; Vector Backbone:pK19; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid expresses a pA-MN fusion protein in E. coli. The MN coding sequence was cloned into pK19SEVII (Giraud-Panis et al., 1995). The pA segment contains 2 IgG binding domains of staphylococcal protein A and was fused to MN with a linker sequence of DDDKEF.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
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