URL: http://www.addgene.org/8606
Proper Citation: RRID:Addgene_8606
Insert Name: barnase, barstar
Organism: Bacillus amyloliquefaciens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:3050134
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Barnase production (secretion)plasmid. Xba1 site is joint between barnase 3' and barstar 5' sequences. Requires an E. coli host carrying the lacI^Q gene. Barnase and barstar genes from B. amyloliquefaciens strain H. 7-117: tac promoter and RBS. 132-194: Structural gene for propeptide phoA signal sequence. 195-628: DNA from B. amyloliquefaciens. 195-527: Structural gene for mature barnase. Genbank #M14442. 528-563: Transcription terminator. 635-1077: DNA from B. amyloliquefaciens. 763-1035: Structural gene for barstar. Genbank #X15545. Grown in Super Broth at 37oC, yield on the order of 20 mg/l is maintained from about 1 to 4 hours after growth stops.
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
Find mentions based on location
{{ ctrl2.mentions.errors.location }}
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pMT413.
No alerts have been found for pMT413.
Source: Addgene