URL: http://www.addgene.org/85968
Proper Citation: RRID:Addgene_85968
Insert Name: sgGFP-NT2
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27984733
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pBA439-based library; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: This library is intended for cloning of individual protospacer sequences into the sgRNA expression cassette followed by isolation and verification of single barcoded expression vectors, as in the paper. It has not been optimized for pooled cloning. Of note, restriction digests indicate the presence of spurious DNA introduced during library construction from pBA439 that has not been observed to carry over into isolated sgRNA expression derivatives. For amplification of the library, the use of high efficiency, electrocompetent cells is strongly advised. To ensure the maintenance of library diversity, calculate your transformation efficiency. 30x coverage of the library is recommended. Also called pBA571
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Source: Addgene