URL: http://www.addgene.org/85389
Proper Citation: RRID:Addgene_85389
Insert Name: ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21175427
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6373; Vector Backbone:p3xFLAG CMV9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A PCR product containing the transmembrane domain of human ICAM-1 was amplified from pCDM8 ICAM-1 (Addgene plasmid # 8632). ecliptic pHluorin (EcpH) was amplified from a Gag-EcpH-expression vector (Sherer et al., Traffic 2003). The EcpH fragment was cloned into p3xFLAG CMV9 using HindIII and EcoRI sites and the ICAM-1 TM fragment was cloned using EcoRI and BamHI.
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Source: Addgene