URL: http://www.addgene.org/85168
Proper Citation: RRID:Addgene_85168
Bacterial Resistance: Kanamycin
Defining Citation: PMID:8529885
Vector Backbone Description: Vector Backbone:pBBR1MCS; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Vector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not controllable with IPTG, due to absence of lacI repressor gene on the plasmid. (chromosomal lacI does not provide sufficient repression). MCS: Has unique KpnI, ApaI, XhoI, HincII, SalI, ClaI, HindIII, EcoRV, EcoRI, SmaI, BamHI, SpeI, XbaI, SacII, SacI sites for cloning
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org