URL: http://www.addgene.org/84266
Proper Citation: RRID:Addgene_84266
Insert Name: RPL1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25159147
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: *Two mutations, R33A and A34E, were introduced into the biotin ligase order to eliminate an unwanted DNA-binding activity without affecting biotinylation, as described in Buoncristiani et al., 1986. Note that the RPL10A protein sequences are identical in human, mouse, and rat, but the human DNA sequence is used in this plasmid.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org