URL: http://www.addgene.org/84232
Proper Citation: RRID:Addgene_84232
Insert Name: Cas9
Organism: S. pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27618190
Vector Backbone Description: Backbone Size:5081; Vector Backbone:GeneArt CRISPR Nuclease vector with OFP reporter; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The four copies of ERT2 are identical in sequence and may recombine occasionally. Hence, it best to use competent cells like STBL3 or NEB Stable to propagate the plasmid and to also check the plasmid size on an agarose gel.
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Source: Addgene