URL: http://www.addgene.org/83480
Proper Citation: RRID:Addgene_83480
Insert Name: Blasticidin S deaminase
Bacterial Resistance: Ampicillin
Defining Citation: PMID:
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:10000; Vector Backbone:lentiCRISPR v2 (#52961); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: lentiCas9-Blast was digested with BamHI and SacII. The resulting small fragment (encoding P2A, blasticidin and a portion of WPRE) was gel-purified. lentiCRISPR v2 was similarly digested and the larger fragment gel-purified. The two fragments combined and ligated.
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Source: Addgene