URL: http://www.addgene.org/79152
Proper Citation: RRID:Addgene_79152
Insert Name: LshC2c2 locus
Organism: Leptotrichia shahii
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:27256883
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pACYC184; Vector Types:; Bacterial Resistance:Chloramphenicol
Comments: *There is a premature stop int he Cas2 protein, removing the final 22 aa's (MKVLAGEVTMGEKISQNKILEF). From the author: "The Cas1/Cas2 are not necessary for the purposes of the plasmid as we have shown that C2c2 is all that is necessary for RNA targeting. [Investigators] should use pC001 if they’re actually interested in the Cas proteins."
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No rating or validation information has been found for pC003 - LshC2C2 locus into pACYC184 for spacer cloning.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org