URL: http://www.addgene.org/78907
Proper Citation: RRID:Addgene_78907
Insert Name: VC3AI
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23857461
Vector Backbone Description: Backbone Size:7378; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Usage information based on sensor VC3AI: VC3AI has no or little background fluorescence, and its activation depends on the cleavage of sensor sequence of DEVDG. In fact, the sensors containing DXXDG other than DEVDG do not work. However, cells expressing VC3AI may have “background” fluorescence in some conditions, and we can conform the so-called basal fluorescence still results from the cleaved VC3AI, because cells could have a low level of activated caspase3/7 in some conditions depending on cell line and culture condition. To avoid the possible detectable basal fluorescence, we infect cells with different dilutions of lentivirus and use the stable cell line expressing sensor protein as high as possible but without basal fluorescence for experiments. Notably, the low level of sensor protein expression work poorly. During our investigation, the basal fluorescence often happens on GC3AI, if the sensor protein expression level is high. This is because GC3AI protein is more stable than other sensors.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org