URL: http://www.addgene.org/78099
Proper Citation: RRID:Addgene_78099
Insert Name: Histone H2B - Citrine
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26912859
Vector Backbone Description: Backbone Marker:Toyobo, Osaka, Japan; Backbone Size:9432; Vector Backbone:pSLR-test; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Can be used for constitutive expression of H2B-citrine. Can be silenced by TetR-KRAB or rTetR-KRAB (using doxycycline). It can be site-specifically integrated in a PhiC31 attP site (the plasmid contains a PhiC31 attB site). The reporter gene is flanked by two full length (1.2kb each) chicken HS4 insulators on each side.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org