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Plasmid Name
RRID:Addgene_75145 RRID Copied  
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RRID:Addgene_75145
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Plasmid Information

URL: http://www.addgene.org/75145

Proper Citation: RRID:Addgene_75145

Insert Name: reprogrammed sortase A variant

Organism: Staphylococcus aureus

Bacterial Resistance: Kanamycin

Defining Citation: PMID:25187567

Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Comments: eSrtA(2A-9) has 51,000 fold change in substrate specificity with activity comparable to eSrtA and it prefers LAXTG substrate over wild-type LPXTG. See sortase A pentamutant (eSrtA) in pET29 for original eSrtA (Addgene plasmid# 75144).

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Data and Source Information

Source: Addgene