URL: http://www.addgene.org/75144
Proper Citation: RRID:Addgene_75144
Insert Name: sortase A pentamutant
Organism: Staphylococcus aureus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21697512
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5370; Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
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Source: Addgene