URL: http://www.addgene.org/70256
Proper Citation: RRID:Addgene_70256
Bacterial Resistance: Kanamycin
Defining Citation: PMID:2160938
Vector Backbone Description: Backbone Size:5941; Vector Backbone:see comments; Vector Types:Conditionally lethal; Bacterial Resistance:Kanamycin
Comments: sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The neomycin/kanamycin determinant in pRL278 was derived from cassette C.K3 (Elhai and Wolk CP, 1988, Gene 68: 119-138). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984 Gene 32: 481-485) and from a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985 Gene 37: 299-304). Additional Reference for this plasmid: Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org