URL: http://www.addgene.org/66850
Proper Citation: RRID:Addgene_66850
Insert Name: WWTR1/TAZ
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21586534
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: I isolated NheI/MfeI fragment from pClneoEGFPC2 TAZ and subcloned into NheI/EcoRi sites of the original pLL3.7. That is the reason why T3 promtoer can be used for the sequencing. That sequence is derived from pClneo. Please note: Plasmid is missing 444bp compared to pLL3.7 backbone sequence. This removes the KS primer and loxP at 5' end of the insert, but is not expected to affect plasmid function.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org