URL: http://www.addgene.org/60524
Proper Citation: RRID:Addgene_60524
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25650551
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: Note that an SfiI digest will affect the Kozak sequence that is already present upstream of the insertion site for the gene of interest. This can be addressed by adding the 3 bases 'ACC' between the 5'-SfiI site and the start codon of your gene of interest: 5'-SfiI---> ggcctctgaggcc ACC atg <---start codon
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Source: Addgene