URL: http://www.addgene.org/53597
Proper Citation: RRID:Addgene_53597
Insert Name: Akt1
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23942361
Vector Backbone Description: Vector Backbone:pHR-IG (pHR’tripCMV-IRES-eGFP); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: AktDN was purchased from Addgene (plasmid # 16243). The AktDN gene is an Akt gene where a point mutation in the 179th amino acid from the N terminal converts a lysine to a methionine. This mutated form of the Akt protein can still be phosphorylated at the S473 site but loses its ability to affect downstream factors.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org