URL: http://www.addgene.org/53281
Proper Citation: RRID:Addgene_53281
Insert Name: crtY, crtI, crtB
Organism: Other
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:17634749
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10609; Vector Backbone:pAC-BETA; Vector Types:Other, low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Expand AllWe found {{ ctrl2.mentions.total_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for pAC-94N.
No alerts have been found for pAC-94N.
Source: Addgene