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Plasmid Name
RRID:Addgene_53281 RRID Copied  
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RRID:Addgene_53281
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Plasmid Information

URL: http://www.addgene.org/53281

Proper Citation: RRID:Addgene_53281

Insert Name: crtY, crtI, crtB

Organism: Other

Bacterial Resistance: Chloramphenicol

Defining Citation: PMID:17634749

Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10609; Vector Backbone:pAC-BETA; Vector Types:Other, low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol

Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

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Data and Source Information

Source: Addgene