URL: http://www.addgene.org/52111
Proper Citation: RRID:Addgene_52111
Insert Name: T cell receptor alpha chain
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17406263
Vector Backbone Description: Backbone Marker:Dario Vignali, Addgene plasmid 52107; Vector Backbone:pMIG II; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells. The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete ‘cleavage’. We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org