URL: http://www.addgene.org/49771
Proper Citation: RRID:Addgene_49771
Insert Name: 35Sp::hCas9
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24112467
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition and placed it under the 35S promoter using the Golden Gate system from S. Marillonnet.
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No rating or validation information has been found for pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST.
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Source: Addgene