URL: http://www.addgene.org/49236
Proper Citation: RRID:Addgene_49236
Insert Name: TALE-TET1CD Backbone
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24108092
Vector Backbone Description: Backbone Marker:Joung lab; Vector Backbone:unknown; Vector Types:TALEN, TALE-TET1 Expression Vector; Bacterial Resistance:Ampicillin
Comments: Location of plasmid features: EF1alpha promoter = 922-2106 NLS = 2118-2142 TALE N-terminal domain = 2167-2577 Clone TALE array repeats between BsmBI sites TALE C-terminal domain = 2665-2949 TET1 catalytic domain = 2956-5123 BGH polyA = 6369-6596
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org