URL: http://www.addgene.org/48868
Proper Citation: RRID:Addgene_48868
Bacterial Resistance: Chloramphenicol and Spectinomycin
Defining Citation: PMID:24376629
Vector Backbone Description: Backbone Size:2673; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Chloramphenicol and Spectinomycin
Comments: This plasmid is designed as destination vector for Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using KpnI and EcoRI. The 3' BsaI overhang can be exchanged using XhoI and XbaI. There is also a a NotI-PacI-SgsI-SfaAI-KpnI MCS in front of the A-overhang and an XbaI-PaeI MCS after the G-overhang. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 640-650bp BsaI site #2 = 2078-2088bp
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Source: Addgene