URL: http://www.addgene.org/48858
Proper Citation: RRID:Addgene_48858
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp BsaI site #2 = 3315-3325bp
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Source: Addgene