URL: http://www.addgene.org/48285
Proper Citation: RRID:Addgene_48285
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:4755; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid is an empty vector to be used with a LIC cloning protocol. It has a TEV cleavable His6 fusion tag on the C-terminus. To clone into this vector, add LIC version 3 fusion tags to the 5' end of your PCR primers. Forward - 5'TTTAAGAAGGAGATATAGTTC3' Reverse - 5'GGATTGGAAGTAGAGGTTCTC3' In addition, ensure that your open reading frame contains an ATG start codon. Linearize the plasmid with HpaI and gel purify. When digesting the DNA with T4 polymerase for LIC version 3, use dGTP for insert and dCTP for vector. Series 9 vectors have BioBrick restriction sites to facilitate subcloning reactions to make polycistronic expression vectors. NotI, PacI, AsiSI, and SbfI are the restriction enzyme sites that flank your open reading frame. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
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No rating or validation information has been found for pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc).
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Source: Addgene